Difference between revisions of "Part:BBa K4583009"

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<partinfo>BBa_K4583009 short</partinfo>
 
<partinfo>BBa_K4583009 short</partinfo>
 
==Usage and Biology==
 
==Usage and Biology==
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===QS system===
 
Queue sensing (QS) is a natural form of cell-cell communication that regulates the metabolic behaviour of bacteria based on changes in their local cell density. As cell density increases, signalling molecules accumulate and are sensed by QS-controlled gene expression regulators, which turn on relevant gene expression.  
 
Queue sensing (QS) is a natural form of cell-cell communication that regulates the metabolic behaviour of bacteria based on changes in their local cell density. As cell density increases, signalling molecules accumulate and are sensed by QS-controlled gene expression regulators, which turn on relevant gene expression.  
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===Esa I/R system===
 
The Esa I/R system is quite special from traditional QS system. The EsaI/R QS system is homologous to the LuxI/R QS system and originates the maize pathogen--<i>Pantoea stewartii</i> subsp. <i>stewartia</i>. EsaR can act as both transcriptional activator and repressor.
 
The Esa I/R system is quite special from traditional QS system. The EsaI/R QS system is homologous to the LuxI/R QS system and originates the maize pathogen--<i>Pantoea stewartii</i> subsp. <i>stewartia</i>. EsaR can act as both transcriptional activator and repressor.
 
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===PesaRwt===
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===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 10:03, 5 October 2023


Wild type promoter of Esa I/R QS system---PesaRwt

Usage and Biology

QS system

Queue sensing (QS) is a natural form of cell-cell communication that regulates the metabolic behaviour of bacteria based on changes in their local cell density. As cell density increases, signalling molecules accumulate and are sensed by QS-controlled gene expression regulators, which turn on relevant gene expression.

Esa I/R system

The Esa I/R system is quite special from traditional QS system. The EsaI/R QS system is homologous to the LuxI/R QS system and originates the maize pathogen--Pantoea stewartii subsp. stewartia. EsaR can act as both transcriptional activator and repressor. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 281
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Referenve

[1]Shong, J., & Collins, C. H. (2013). Engineering the esaR promoter for tunable quorum sensing- dependent gene expression. ACS synthetic biology, 2(10), 568–575. https://doi.org/10.1021/sb4000433 [2]Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. Plasmid, 109, 102491. https://doi.org/10.1016/j.plasmid.2020.102491