Difference between revisions of "Part:BBa K4765122"

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===Introduction===
 
===Introduction===
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This composite part is designed for the production of exopolysaccharide(EPS), and detection of EPS’s adhesion ability. It includes ''E. coli pgmA'' on the downstream site and ''E. coli galU'' on the upstream site, two essential enzymes in EPS biosynthesis pathway. These two enzymes are tested by iGEM20_XJTU-China in [https://parts.igem.org/Part:BBa_K3331001 BBa_K3331001(galU)]and [https://parts.igem.org/Part:BBa_K3331002 BBa_K3331002(pgmA)]. The following mScarlet is utilized to detection E. coli with adhesion ability.
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This composite part is designed for the production of exopolysaccharide(EPS), and detection of EPS’s adhesion ability. It includes ''E. coli pgmA'' on the downstream site and ''E. coli galU'' on the upstream site, two essential enzymes in EPS biosynthesis pathway. These two enzymes are tested by iGEM20_XJTU-China in [https://parts.igem.org/Part:BBa_K3331001 BBa_K3331001(galU)]and [https://parts.igem.org/Part:BBa_K3331002 BBa_K3331002(pgmA)]. The following mScarlet is utilized to detect ''E. coli'' 's adhesion ability.
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 09:28, 5 October 2023


ribozyme connected: pgmA + galU + mScarlet contributed by Fudan iGEM 2023


Introduction

This composite part is designed for the production of exopolysaccharide(EPS), and detection of EPS’s adhesion ability. It includes E. coli pgmA on the downstream site and E. coli galU on the upstream site, two essential enzymes in EPS biosynthesis pathway. These two enzymes are tested by iGEM20_XJTU-China in BBa_K3331001(galU)and BBa_K3331002(pgmA). The following mScarlet is utilized to detect E. coli 's adhesion ability.

Usage and Biology

We use this part to improve EPS production of E. coli and assessment of EPS’s adhesion ability.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3285
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]