Difference between revisions of "Part:BBa K4765121"
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===Introduction=== | ===Introduction=== | ||
| − | This composite part is designed for the production of exopolysaccharide (EPS), and detection of EPS’s adhesion ability. It includes ''E. coli'' ''galU'' on the upstream site and ''E. coli'' ''pgmA'' on the downstream site, two essential enzymes in EPS biosynthesis pathway <ref>需要参考文献</ref>. These two enzymes were tested by iGEM20_XJTU-China in [https://parts.igem.org/Part:BBa_K3331001 K3331001(galU)]and [https://parts.igem.org/Part:BBa_K3331002 K3331002(pgmA)]. The following mScarlet is utilized to | + | This composite part is designed for the production of exopolysaccharide (EPS), and detection of EPS’s adhesion ability. It includes ''E. coli'' ''galU'' on the upstream site and ''E. coli'' ''pgmA'' on the downstream site, two essential enzymes in EPS biosynthesis pathway <ref>需要参考文献</ref>. These two enzymes were tested by iGEM20_XJTU-China in [https://parts.igem.org/Part:BBa_K3331001 K3331001(galU)]and [https://parts.igem.org/Part:BBa_K3331002 K3331002(pgmA)]. The following mScarlet is utilized to detect ''E. coli'' 's adhesion ability. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 09:27, 5 October 2023
ribozyme connected: galU + pgmA + mScarlet
Introduction
This composite part is designed for the production of exopolysaccharide (EPS), and detection of EPS’s adhesion ability. It includes E. coli galU on the upstream site and E. coli pgmA on the downstream site, two essential enzymes in EPS biosynthesis pathway [1]. These two enzymes were tested by iGEM20_XJTU-China in K3331001(galU)and K3331002(pgmA). The following mScarlet is utilized to detect E. coli 's adhesion ability.
Usage and Biology
We use this part to improve EPS production of E. coli and assessment of EPS’s adhesion ability.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 3285
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ 需要参考文献