Difference between revisions of "Part:BBa K4887011:Design"

 
 
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===Design Notes===
 
===Design Notes===
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The GUS gene was used as a reporter gene harbored in pCAMBIA1301 to indicate whether the expression vector of sgRNA (IbGBSSI) wan successfully transformed in to sweet potato lines.
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The GUS gene was used as a reporter gene harbored in pCAMBIA1301s to indicate whether the expression vector of sgRNA (IbGBSSI) (BBa_K4887002) was successfully transformed in to sweet potato lines.
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Latest revision as of 06:38, 5 October 2023


GUS reporter for plant expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1819
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The GUS gene was used as a reporter gene harbored in pCAMBIA1301s to indicate whether the expression vector of sgRNA (IbGBSSI) (BBa_K4887002) was successfully transformed in to sweet potato lines.


Source

The gus reporter gene encodes beta-glucuronidase (GUS), an enzyme originated from Escherichia coli.

References