Difference between revisions of "Part:BBa K4887003:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | It is the FLAG geng harboured in the backbone of plasmid psgR-Cas9-At. | + | It is the FLAG geng harboured in the backbone of plasmid psgR-Cas9-At.The transgenic lines can be detected by western blot using FLAG antibody. |
| − | + | ||
===Source=== | ===Source=== | ||
| + | 3*FLAG is synthesized according to Wenshan Liu et al. (2015) | ||
| − | |||
===References=== | ===References=== | ||
| + | Wenshan Liu, Xiaohong Zhu, Mingguang Lei, Qingyou Xia, JR Botella, Jian-Kang Zhu, Yanfei Mao. A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis. Science Bulletin, 2015, 15(60). | ||
Latest revision as of 06:13, 5 October 2023
3*FLAG
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It is the FLAG geng harboured in the backbone of plasmid psgR-Cas9-At.The transgenic lines can be detected by western blot using FLAG antibody.
Source
3*FLAG is synthesized according to Wenshan Liu et al. (2015)
References
Wenshan Liu, Xiaohong Zhu, Mingguang Lei, Qingyou Xia, JR Botella, Jian-Kang Zhu, Yanfei Mao. A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis. Science Bulletin, 2015, 15(60).