Difference between revisions of "Part:BBa K4907000"
HonglinSong (Talk | contribs) |
(→Biology) |
||
| Line 5: | Line 5: | ||
===Biology=== | ===Biology=== | ||
| + | https://static.igem.wiki/teams/4907/wiki/parts/k4907000.png | ||
====sfibp==== | ====sfibp==== | ||
SfIBP is a kind of antifreeze proteins (AFPs) found from <i>Shewanella frigidimarina</i> (1). It is capable to prevent liquid from freezing within a certain temperature range and inhibit ice growth, which is defined as <b>Thermal Hysteresis (TH)</b> and <b>ice recrystallization inhibition (IRI)</b> respectively. Additionally, the IRI activity of SfIBP is much higher beyond other AFPs. | SfIBP is a kind of antifreeze proteins (AFPs) found from <i>Shewanella frigidimarina</i> (1). It is capable to prevent liquid from freezing within a certain temperature range and inhibit ice growth, which is defined as <b>Thermal Hysteresis (TH)</b> and <b>ice recrystallization inhibition (IRI)</b> respectively. Additionally, the IRI activity of SfIBP is much higher beyond other AFPs. | ||
| + | |||
===Usage and Design=== | ===Usage and Design=== | ||
In order to measure TH and IRI activity of SfIBP, and finally verify if it is capable to solve soil frost problem, a His-tag (6×his) was added to the C-terminal of SfIBP for purification. We constructed this part and assembled it on the expression vector pET-28a(+). | In order to measure TH and IRI activity of SfIBP, and finally verify if it is capable to solve soil frost problem, a His-tag (6×his) was added to the C-terminal of SfIBP for purification. We constructed this part and assembled it on the expression vector pET-28a(+). | ||
Revision as of 04:56, 5 October 2023
sfibp-his tag
Biology
sfibp
SfIBP is a kind of antifreeze proteins (AFPs) found from Shewanella frigidimarina (1). It is capable to prevent liquid from freezing within a certain temperature range and inhibit ice growth, which is defined as Thermal Hysteresis (TH) and ice recrystallization inhibition (IRI) respectively. Additionally, the IRI activity of SfIBP is much higher beyond other AFPs.
Usage and Design
In order to measure TH and IRI activity of SfIBP, and finally verify if it is capable to solve soil frost problem, a His-tag (6×his) was added to the C-terminal of SfIBP for purification. We constructed this part and assembled it on the expression vector pET-28a(+).
123
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 634
Illegal AgeI site found at 649 - 1000COMPATIBLE WITH RFC[1000]