Difference between revisions of "Part:BBa K4621020"
 
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gadB is the coding sequence for glutamate decarboxylase, GAD is a pyridoxal 5'phosphate (PLP)-dependent decarboxylase widespread in bacteria yeast and filamentous fungi. The carboxyl group (-COOH) on the catalytic glutamate molecule is released to CO2, and 4-Aminobutanoate is generated at the same time, which participates in the utilization of α-Ketoglutaricacid in the TCA cycle. The specific synthesis route is shown in FIG. 1.
 
gadB is the coding sequence for glutamate decarboxylase, GAD is a pyridoxal 5'phosphate (PLP)-dependent decarboxylase widespread in bacteria yeast and filamentous fungi. The carboxyl group (-COOH) on the catalytic glutamate molecule is released to CO2, and 4-Aminobutanoate is generated at the same time, which participates in the utilization of α-Ketoglutaricacid in the TCA cycle. The specific synthesis route is shown in FIG. 1.
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https://static.igem.wiki/teams/4621/wiki/parts/biosynthetic-pathway-for-gaba.jpg
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Fig.1 Biosynthetic pathway of GABA
 
Fig.1 Biosynthetic pathway of GABA
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Modified Streptomyces SCUT-3. They were then inoculated in a 40 mL LB medium system. After fermentation at 40 ° C and 220 rpm for 72 hours, the product GABA was measured, and the results were as follows:
 
Modified Streptomyces SCUT-3. They were then inoculated in a 40 mL LB medium system. After fermentation at 40 ° C and 220 rpm for 72 hours, the product GABA was measured, and the results were as follows:
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https://static.igem.wiki/teams/4621/wiki/parts/fermentation-data-of-wt-gaba-gi9.png
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[WT GABA Gi1 3 9 fermentation data graph]
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Fig.2fermentation data of WT,GABA,Gi9
  
  

Latest revision as of 19:07, 4 October 2023


gadB is the coding sequence of glutamate decarboxylase, which converts glutamate to 4-Aminobutanoate


Usage, Biology and Characterization：

gadB is the coding sequence for glutamate decarboxylase, GAD is a pyridoxal 5'phosphate (PLP)-dependent decarboxylase widespread in bacteria yeast and filamentous fungi. The carboxyl group (-COOH) on the catalytic glutamate molecule is released to CO2, and 4-Aminobutanoate is generated at the same time, which participates in the utilization of α-Ketoglutaricacid in the TCA cycle. The specific synthesis route is shown in FIG. 1. biosynthetic-pathway-for-gaba.jpg


Fig.1 Biosynthetic pathway of GABA


Modified Streptomyces SCUT-3. They were then inoculated in a 40 mL LB medium system. After fermentation at 40 ° C and 220 rpm for 72 hours, the product GABA was measured, and the results were as follows: fermentation-data-of-wt-gaba-gi9.png


Fig.2fermentation data of WT,GABA,Gi9



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
    Illegal NgoMIV site found at 1242
    Illegal AgeI site found at 470
    Illegal AgeI site found at 690
  • 1000
    COMPATIBLE WITH RFC[1000]