Difference between revisions of "Part:BBa K4621099"
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Paired with Ect-R, EctOperon can be amplified by PCR from the genome of strain SCUT-3 discovered in our laboratory,inserted into the pSET152PZ plasmid and connected to the promoter P24880. ectABCD-F and Ect-R primers amplified a fragment length of 3508 bp containing EctOperon. | Paired with Ect-R, EctOperon can be amplified by PCR from the genome of strain SCUT-3 discovered in our laboratory,inserted into the pSET152PZ plasmid and connected to the promoter P24880. ectABCD-F and Ect-R primers amplified a fragment length of 3508 bp containing EctOperon. | ||
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Latest revision as of 18:59, 4 October 2023
Used to amplify EctOperon fragments
Paired with Ect-R, EctOperon can be amplified by PCR from the genome of strain SCUT-3 discovered in our laboratory,inserted into the pSET152PZ plasmid and connected to the promoter P24880. ectABCD-F and Ect-R primers amplified a fragment length of 3508 bp containing EctOperon.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]