Difference between revisions of "Part:BBa K260009:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K260009 short</partinfo>
 
<partinfo>BBa_K260009 short</partinfo>
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===Design Notes===
 
===Design Notes===
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The 2x 70 bp homologies were designed to allow "pseudo-cloning" of a BioBrick from the present backbone '''pMA-RQ-@TetFlp''' to pTetFlp [[Part:BBa_K260002|]] via recombineering  using the PvuII fragment containing the BioBrick, where BioBrick prefix and suffix are kept untouched.
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The 2x 70 bp homologies were designed to allow "pseudo-cloning" of a BioBrick from the present backbone '''pMA-RQ-@TetFlp''' to pTetFlp [[Part:BBa_K260002|BBa_K260002]] via recombineering  using the PvuII fragment containing the BioBrick, where BioBrick prefix and suffix are kept untouched.
  
  
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===Source===
 
===Source===
  
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Geneart plasmid pMA-RQ, with 2x 70 bp from pTetFlp [[Part:BBa_K260002|]].
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Geneart plasmid pMA-RQ, with 2x 70 bp from pTetFlp [[Part:BBa_K260002|BBa_K260002]].
  
 
===References===
 
===References===

Latest revision as of 08:07, 18 October 2009

pMA-RQ-@TetFlp


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2578
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2584
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2578
    Illegal XhoI site found at 61
    Illegal XhoI site found at 2572
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2578
    Illegal suffix found at 2
    Illegal XbaI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2578
    Plasmid lacks a suffix.
    Illegal XbaI site found at 67
    Illegal XbaI site found at 2593
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

The 2x 70 bp homologies were designed to allow "pseudo-cloning" of a BioBrick from the present backbone pMA-RQ-@TetFlp to pTetFlp BBa_K260002 via recombineering using the PvuII fragment containing the BioBrick, where BioBrick prefix and suffix are kept untouched.


Source

Geneart plasmid pMA-RQ, with 2x 70 bp from pTetFlp BBa_K260002.

References