Difference between revisions of "Part:BBa K4882002"
 
Line 4: Line 4:
  
 
The Mcl1 signal peptide (Mcl1ss) is from the collagen-like protein MCL1 of M. anisopliae. Mcl1ss is an 18-amino acid secretory signal peptide at the N-terminus of the MCL1 protein (Wang & St Leger, 2006).
 
The Mcl1 signal peptide (Mcl1ss) is from the collagen-like protein MCL1 of M. anisopliae. Mcl1ss is an 18-amino acid secretory signal peptide at the N-terminus of the MCL1 protein (Wang & St Leger, 2006).
−
 
−
<!-- Add more about the biology of this part here-->
 
−
===Usage and Biology===
 
−
 
−
To improve the efficiency of M. anisopliae as a fungal biopesticide, we choose to introduce the LqhIT2 toxin into the fungus. To promote the secretion of the toxin, a Mcl1ss is linked at the 5’ end of the LqhIT2 gene.
 
  
 
<!-- -->
 
<!-- -->
Line 19: Line 14:
 
<partinfo>BBa_K4882002 parameters</partinfo>
 
<partinfo>BBa_K4882002 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
<!-- Add more about the biology of this part here-->
 +
===Usage and Biology===
 +
 +
To improve the efficiency of M. anisopliae as a fungal biopesticide, we choose to introduce the LqhIT2 toxin into the fungus. To promote the secretion of the toxin, a Mcl1ss is linked at the 5’ end of the LqhIT2 gene.
  
 
==References==
 
==References==
 
Wang C, St Leger RJ. A collagenous protective coat enables Metarhizium anisopliae to evade insect immune responses. Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6647-52. doi: 10.1073/pnas.0601951103. Epub 2006 Apr 13. PMID: 16614065; PMCID: PMC1458935.
 
Wang C, St Leger RJ. A collagenous protective coat enables Metarhizium anisopliae to evade insect immune responses. Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6647-52. doi: 10.1073/pnas.0601951103. Epub 2006 Apr 13. PMID: 16614065; PMCID: PMC1458935.

Latest revision as of 14:48, 4 October 2023


MCL1 signal peptide from Metarhizium anisopliae

The Mcl1 signal peptide (Mcl1ss) is from the collagen-like protein MCL1 of M. anisopliae. Mcl1ss is an 18-amino acid secretory signal peptide at the N-terminus of the MCL1 protein (Wang & St Leger, 2006).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

To improve the efficiency of M. anisopliae as a fungal biopesticide, we choose to introduce the LqhIT2 toxin into the fungus. To promote the secretion of the toxin, a Mcl1ss is linked at the 5’ end of the LqhIT2 gene.

References

Wang C, St Leger RJ. A collagenous protective coat enables Metarhizium anisopliae to evade insect immune responses. Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6647-52. doi: 10.1073/pnas.0601951103. Epub 2006 Apr 13. PMID: 16614065; PMCID: PMC1458935.