Difference between revisions of "Part:BBa K223051"
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<partinfo>BBa_K223051 short</partinfo> | <partinfo>BBa_K223051 short</partinfo> | ||
| − | This is a modified trp operon, promoter and operator site, that recognizes and binds to a doubly mutant repressor | + | This is a modified trp operon, promoter and operator site, that recognizes and binds to a doubly mutant repressor and its corepressor, 5-methyl-L-tryptophan. In conjunction with the mutant tryptophan repressor, this modified operon functions as an inverter, converting decreasing levels of 5MT into a PoPs output signal for downstream transcription. |
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Latest revision as of 07:33, 18 October 2009
5MT operon (promoter-operator)
This is a modified trp operon, promoter and operator site, that recognizes and binds to a doubly mutant repressor and its corepressor, 5-methyl-L-tryptophan. In conjunction with the mutant tryptophan repressor, this modified operon functions as an inverter, converting decreasing levels of 5MT into a PoPs output signal for downstream transcription.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 149 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 150
Illegal PstI site found at 164
Illegal NotI site found at 7
Illegal NotI site found at 157 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 150 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 150
Illegal PstI site found at 164 - 1000COMPATIBLE WITH RFC[1000]