Difference between revisions of "Part:BBa K4743030"
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<span class='h3bb'><big>'''Kill switch’s mechanism'''</big></span> | <span class='h3bb'><big>'''Kill switch’s mechanism'''</big></span> | ||
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The graph below (Figure 1) shows the first exposure to the normal condition. When there is a low amount of hydrogen sulfide, the translated sqrR protein (red) represses the sqr promoter, preventing the transcription of recombinase DRE or CRE and the antitoxin . Additionally, the terminator labeled by rox sequences halts the transcription of toxin. Consequently, no death occurs because there is no toxin and antitoxin present. | The graph below (Figure 1) shows the first exposure to the normal condition. When there is a low amount of hydrogen sulfide, the translated sqrR protein (red) represses the sqr promoter, preventing the transcription of recombinase DRE or CRE and the antitoxin . Additionally, the terminator labeled by rox sequences halts the transcription of toxin. Consequently, no death occurs because there is no toxin and antitoxin present. | ||
| − | <html><img style="width: | + | <html><img style="width:800px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/kill-switch-first-exposure.png" </html> |
<center>Figure 1: First exposure to the normal condition. </center> | <center>Figure 1: First exposure to the normal condition. </center> | ||
The second condition is exposure to the gut environment(shown in figure 2). When the organism is exposed to the gut, which hydrogen sulfide(Blue) presents , the suppressive effect of the sqrR suppressor decreases, resulting in the transcription of recombinase DRE or CRE and the antitoxin .The recombinase DRE targets the terminator sequence labeled by rox and cleaves it, allowing the toxin to be transcribed. However, the transcribed antitoxin neutralizes the toxin and inhibits its toxic activity. In conclusion, the cell remains alive. | The second condition is exposure to the gut environment(shown in figure 2). When the organism is exposed to the gut, which hydrogen sulfide(Blue) presents , the suppressive effect of the sqrR suppressor decreases, resulting in the transcription of recombinase DRE or CRE and the antitoxin .The recombinase DRE targets the terminator sequence labeled by rox and cleaves it, allowing the toxin to be transcribed. However, the transcribed antitoxin neutralizes the toxin and inhibits its toxic activity. In conclusion, the cell remains alive. | ||
| − | <html><img style="width: | + | <html><img style="width:800px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/kill-switch-in-gut-environment.png" </html> |
<center>Figure 2: exposure to the gut environment. </center> | <center>Figure 2: exposure to the gut environment. </center> | ||
The third condition(Figure 3)represents the organism's second exposure to the normal environment, which signifies its escape from the gut or excretion of feces. In this condition, the sqrR suppressor binds to the promoter once again, restricting transcription of antitoxin. However, the terminator that halts toxin transcription is excised, resulting in the transcription of the toxin. In this scenario, the bacteria lose the antitoxin, ultimately leading to cell death. | The third condition(Figure 3)represents the organism's second exposure to the normal environment, which signifies its escape from the gut or excretion of feces. In this condition, the sqrR suppressor binds to the promoter once again, restricting transcription of antitoxin. However, the terminator that halts toxin transcription is excised, resulting in the transcription of the toxin. In this scenario, the bacteria lose the antitoxin, ultimately leading to cell death. | ||
| − | <html><img style="width: | + | <html><img style="width:800px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/kill-switch-second-exposure.png" </html> |
<center>Figure 3: Second exposure to the normal environment. </center> | <center>Figure 3: Second exposure to the normal environment. </center> | ||
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<span class='h3bb'><big>'''Sequence and Features'''</big></span> | <span class='h3bb'><big>'''Sequence and Features'''</big></span> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
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Revision as of 14:01, 4 October 2023
Bacillus subtilis Hydrogen sulfide based kill switch
Kill switch’s mechanism
The graph below (Figure 1) shows the first exposure to the normal condition. When there is a low amount of hydrogen sulfide, the translated sqrR protein (red) represses the sqr promoter, preventing the transcription of recombinase DRE or CRE and the antitoxin . Additionally, the terminator labeled by rox sequences halts the transcription of toxin. Consequently, no death occurs because there is no toxin and antitoxin present.
The second condition is exposure to the gut environment(shown in figure 2). When the organism is exposed to the gut, which hydrogen sulfide(Blue) presents , the suppressive effect of the sqrR suppressor decreases, resulting in the transcription of recombinase DRE or CRE and the antitoxin .The recombinase DRE targets the terminator sequence labeled by rox and cleaves it, allowing the toxin to be transcribed. However, the transcribed antitoxin neutralizes the toxin and inhibits its toxic activity. In conclusion, the cell remains alive.
The third condition(Figure 3)represents the organism's second exposure to the normal environment, which signifies its escape from the gut or excretion of feces. In this condition, the sqrR suppressor binds to the promoter once again, restricting transcription of antitoxin. However, the terminator that halts toxin transcription is excised, resulting in the transcription of the toxin. In this scenario, the bacteria lose the antitoxin, ultimately leading to cell death.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 464
Illegal AgeI site found at 1623 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 677