Difference between revisions of "Part:BBa K4882006"

 
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<partinfo>BBa_K4882006 short</partinfo>
 
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This part consists of a truncated Pmcl1 hemolymph inducible promoter, an MCL1 signal peptide linked LqhIT2 insect-specific toxin, and a trpC terminator.
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This is a complete expression cassette consisting of a hemolymph inducible promoter Pmc1(short), a Mcl1 secretory signal peptide fused with an insect-specific toxin LqhIT2, and a trpC terminator.  
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===Usage and Biology===
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<partinfo>BBa_K4882006 parameters</partinfo>
 
<partinfo>BBa_K4882006 parameters</partinfo>
 
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===Usage and Biology===
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Metarhizium anisopliae is an entomopathogenic fungus widely used as a biopesticide. However, it is less efficient than most chemical pesticides. To improve the efficiency of M. anisopliae as a fungal biopesticide, we choose to introduce the LqhIT2 toxin into the fungus. To promote the secretion of the toxin, a Mcl1ss is linked at the 5’ end of the LqhIT2 gene. We put Mcl1ss-LqhIT2 (BBa_K4882007) downstream of Pmcl1 (short, [https://parts.igem.org/Part:BBa_K4882000 BBa_K4882000]). This design limited the expression of LqhIT2 inside the insect body and improved the biosafety of our product. A trpC terminator was also included to form the complete expression cassette.

Revision as of 13:54, 4 October 2023


Pmcl1 (short)-MCL1ss-LqhIT2-TtrpC

This is a complete expression cassette consisting of a hemolymph inducible promoter Pmc1(short), a Mcl1 secretory signal peptide fused with an insect-specific toxin LqhIT2, and a trpC terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 489
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1731
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 45
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

Metarhizium anisopliae is an entomopathogenic fungus widely used as a biopesticide. However, it is less efficient than most chemical pesticides. To improve the efficiency of M. anisopliae as a fungal biopesticide, we choose to introduce the LqhIT2 toxin into the fungus. To promote the secretion of the toxin, a Mcl1ss is linked at the 5’ end of the LqhIT2 gene. We put Mcl1ss-LqhIT2 (BBa_K4882007) downstream of Pmcl1 (short, BBa_K4882000). This design limited the expression of LqhIT2 inside the insect body and improved the biosafety of our product. A trpC terminator was also included to form the complete expression cassette.