Difference between revisions of "Part:BBa K4882001"
| Line 9: | Line 9: | ||
<figure> | <figure> | ||
<div class = "center"> | <div class = "center"> | ||
| − | <center><img src = "https://static.igem.wiki/teams/4882/wiki/part-lqhit2.png" style = "width: | + | <center><img src = "https://static.igem.wiki/teams/4882/wiki/part-lqhit2.png" style = "width:280px"></center> |
</div> | </div> | ||
| − | <figcaption><center> | + | <figcaption><center>Figure 1. The 3D structure of LqhIT2 (PDB ID: 2I61; Karbat et al., 2007). </center></figcaption> |
</figure> | </figure> | ||
</body> | </body> | ||
Revision as of 12:49, 4 October 2023
LqhIT2, an insect-specific toxin from Leiurus quinquestriatus hebraeus
LqhIT2 is a 61 amino acid-long scorpion depressant toxin from L. quinquestriatus hebraeus. Although the scorpion is dangerous to humans, LqhIT2 is not. LqhIT2 shows a high preference for insect voltage-gated sodium channels and is totally harmless to mammals (Karbat et al., 2007; Zlotkin et al., 1991).
Usage and Biology
Since LqhIT2 is only toxic to insects, it is a good choice for pesticides. To improve the efficiency and effectiveness of Metarhizium anisopliae as a fungal biopesticide, we chose to introduce LqhIT2 into the fungus. LqhIT2 significantly improved the virulence of M. anisopliae against pests.
References
Karbat, I.; Turkov, M.; Cohen, L.; Kahn, R.; Gordon, D.; Gurevitz, M.; Frolow, F. X-Ray Structure and Mutagenesis of the Scorpion Depressant Toxin LqhIT2
Zlotkin E, Eitan M, Bindokas VP, Adams ME, Moyer M, Burkhart W, Fowler E. Functional duality and structural uniqueness of depressant insect-selective neurotoxins. Biochemistry. 1991 May 14;30(19):4814-21. doi: 10.1021/bi00233a025. PMID: 2029523.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 88
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]