Difference between revisions of "Part:BBa K4583000"

(→‎Protocols)
(→‎Protocols)
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==Characterization==
 
==Characterization==
 
===Protocols===
 
===Protocols===
 +
====Experiment Conditions====
 
* <em>E. coli</em> MG1655
 
* <em>E. coli</em> MG1655
 
* 30<sup>o</sup>C, 48h
 
* 30<sup>o</sup>C, 48h
 
* Equipment:
 
* Equipment:
 +
====Data Curation====
  
 
===Characterization using fluorescent proteins===
 
===Characterization using fluorescent proteins===
 
===Comparison of expression time with cell density===
 
===Comparison of expression time with cell density===

Revision as of 11:55, 4 October 2023

PYU3

PYU3 is the promoter of the gene orf-0464, and it comes from Escherichia coli. It will express at the late stationary phase.

Usage and Biology

When the bacteria enter the stationary phase, the physiological state of the bacteria changes significantly. During this phase, many genes will respond to make timely adjustments. This part (BBa_K4583000) is the promoter of the gene orf-0464. Its most notable feature is that it will be expressed in the late stationary phase. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

Protocols

Experiment Conditions

  • E. coli MG1655
  • 30oC, 48h
  • Equipment:

Data Curation

Characterization using fluorescent proteins

Comparison of expression time with cell density