Difference between revisions of "Part:BBa K4800001"
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    <p style="font-size: 160%; font-weight: bold;">Result:
 
 
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    <p style="font-size: 160%; font-weight: bold;">Adjusting the distance between FNR binding site and the -35 region of
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     <p><img src="https://static.igem.wiki/teams/4800/wiki/parts/mmcar.png" width="60%" height="60%"></p>
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        promoter vgb fine tunes the inhibitory effect of oxygen on the promoter.
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     <p><img src="https://static.igem.wiki/teams/4800/wiki/parts/mmcar.png" width="80%" height="80%"></p>
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         <strong>Fig.9 Construction of the recombinant plasmid for pMTL-PvgbF7-bs2</strong>
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         <strong>Fig.1 Catalytic pocket for MmCAR</strong>
 
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     <p>MmCAR is a carboxylic acid reductase from marine bacteria. During the experiment, we mutated the original 302nd amino
 
     <p>MmCAR is a carboxylic acid reductase from marine bacteria. During the experiment, we mutated the original 302nd amino
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       the original one
 
       the original one
 
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<p style="font-size: 160%; font-weight: bold;">Result:
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    <p><img src="https://static.igem.wiki/teams/4800/wiki/parts/q302e.png" width="80%" height="80%"></p>
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        <strong>Fig.2 Q302E and MmCAR produce 1,5-PDO in KA30</strong>
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    <p>Through fermentation experiments, we have verified that Q302E can produce more pentanediol
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4119080 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4800001 SequenceAndFeatures</partinfo>

Revision as of 11:45, 4 October 2023


rrnB T1 terminator

Carboxylate reductase from Mycobacterium marinum(Mutation of glutamine at position 302 to glutamate)



Document

Fig.1 Catalytic pocket for MmCAR

MmCAR is a carboxylic acid reductase from marine bacteria. During the experiment, we mutated the original 302nd amino acid glutamine to glutamic acid through acid-base mutation. The mutated Q302E has a significant improvement compared to the original one

Result:

Fig.2 Q302E and MmCAR produce 1,5-PDO in KA30

Through fermentation experiments, we have verified that Q302E can produce more pentanediol

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]