Difference between revisions of "Part:BBa K4653001"
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===Biology=== | ===Biology=== | ||
− | Chitin synthase (CHS) catalyzes the synthesis of chitin, a major structural component of fungal cell walls consisting of β-1, 4-linked N-acetylglucosamine polymers. CHS can be divided into seven classes, and ascomycetes usually have representatives of all seven classes, with each enzyme having a specific role. CHS (III, V, and VI) are specific to molds, suggesting that they may play an important role in hyphal growth. | + | Chitin synthase (CHS) catalyzes the synthesis of chitin, a major structural component of fungal cell walls consisting of β-1, 4-linked N-acetylglucosamine polymers. CHS can be divided into seven classes, and ascomycetes usually have representatives of all seven classes, with each enzyme having a specific role. CHS (III, V, and VI) are specific to molds, suggesting that they may play an important role in hyphal growth. Like other ascomycetes, <i>B. cinerea</i> contains two CHSIII genes. It has been shown that the <i>BcchsIIIa</i> gene is most expressed in the CHSIII genes, whereas the <i>BcchsIIIb</i> gene is not expressed in any of the growth conditions used. By silencing the important chitin synthase gene BcCHSIIIa, the formation of the cell wall of <i>B. cinerea</i> could be affected, thus killing pathogenic fungi. |
− | By silencing the important chitin synthase gene BcCHSIIIa, the formation of the cell wall of B. cinerea could be affected, thus killing pathogenic fungi. | + | |
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Revision as of 07:50, 4 October 2023
shRNA(CHSIIIa)-1
To kill B. cinerea, we designed two pieces of shRNAs targeting the CHSIIIa gene of the pathogen, which is essential for its cell wall formation, based on RNAi technology.
Sequencing
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Biology
Chitin synthase (CHS) catalyzes the synthesis of chitin, a major structural component of fungal cell walls consisting of β-1, 4-linked N-acetylglucosamine polymers. CHS can be divided into seven classes, and ascomycetes usually have representatives of all seven classes, with each enzyme having a specific role. CHS (III, V, and VI) are specific to molds, suggesting that they may play an important role in hyphal growth. Like other ascomycetes, B. cinerea contains two CHSIII genes. It has been shown that the BcchsIIIa gene is most expressed in the CHSIII genes, whereas the BcchsIIIb gene is not expressed in any of the growth conditions used. By silencing the important chitin synthase gene BcCHSIIIa, the formation of the cell wall of B. cinerea could be affected, thus killing pathogenic fungi.