Difference between revisions of "Part:BBa K4634004"

 
 
Line 9: Line 9:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
===Sequence and Features===
 +
<span class='h3bb'></span>
 
<partinfo>BBa_K4634004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4634004 SequenceAndFeatures</partinfo>
  

Latest revision as of 04:40, 4 October 2023


Hypoxia-Induced Promoter Experiment: Control Group

This is a reporter system that serves as a control group in experiments with hypoxia-inducible promoters, compared to part BBa_K4634003. This composite part construct of a pET28a(+) backbone and mcherry. mcherry is controlled by T7 promoter and lac operator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5242
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5242
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5248
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5242
    Illegal BglII site found at 5130
    Illegal XhoI site found at 22
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5242
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5242
    Illegal XbaI site found at 5257
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 303
    Illegal NgoMIV site found at 3350
    Illegal NgoMIV site found at 3510
    Illegal NgoMIV site found at 5098
    Illegal AgeI site found at 5951
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2429