Difference between revisions of "Part:BBa K4634002"
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===pET28a(+) backbone=== | ===pET28a(+) backbone=== | ||
The pET28a vector is a molecular biology tool specifically designed for the efficient expression of recombinant proteins in bacterial host cells. This vector includes the T7 promoter and lac operator, it is a vector used to construct expression systems. Additionally, the vector carries a resistance marker, typically kanamycin resistance, for the selection of transformed bacterial cells, and a high-copy-number origin of replication compatible with Escherichia coli, ensuring efficient plasmid replication. Other than that, this plasmid backbone contain a rop protein which maintains plasmids at low copy number. | The pET28a vector is a molecular biology tool specifically designed for the efficient expression of recombinant proteins in bacterial host cells. This vector includes the T7 promoter and lac operator, it is a vector used to construct expression systems. Additionally, the vector carries a resistance marker, typically kanamycin resistance, for the selection of transformed bacterial cells, and a high-copy-number origin of replication compatible with Escherichia coli, ensuring efficient plasmid replication. Other than that, this plasmid backbone contain a rop protein which maintains plasmids at low copy number. | ||
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| + | <!-- --> | ||
| + | ===Sequence and Features=== | ||
| + | <span class='h3bb'></span> | ||
| + | <partinfo>BBa_K4634001 SequenceAndFeatures</partinfo> | ||
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| + | <!-- Uncomment this to enable Functional Parameter display | ||
| + | ===Functional Parameters=== | ||
| + | <partinfo>BBa_K4634001 parameters</partinfo> | ||
| + | <!-- --> | ||
Latest revision as of 04:24, 4 October 2023
pET28a(+) backbone
The pET28a vector is a molecular biology tool specifically designed for the efficient expression of recombinant proteins in bacterial host cells. This vector includes the T7 promoter and lac operator, it is a vector used to construct expression systems. Additionally, the vector carries a resistance marker, typically kanamycin resistance, for the selection of transformed bacterial cells, and a high-copy-number origin of replication compatible with Escherichia coli, ensuring efficient plasmid replication. Other than that, this plasmid backbone contain a rop protein which maintains plasmids at low copy number.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]