Difference between revisions of "Part:BBa K4897002"
| Line 3: | Line 3: | ||
<partinfo>BBa_K4897002 short</partinfo> | <partinfo>BBa_K4897002 short</partinfo> | ||
| − | BS DNA- | + | BS DNA-322 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. Binding region is the key element in reacting with P. acne 16s rRNA gene. The DNA ligase will perform the ligation of the single strand DNA meanwhile the phi29 will generate double stranded DNA through amplification primers |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 02:40, 4 October 2023
BS DNA-322 (BS DNA 3.0) using in L-RCA for detecting P. acne
BS DNA-322 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. Binding region is the key element in reacting with P. acne 16s rRNA gene. The DNA ligase will perform the ligation of the single strand DNA meanwhile the phi29 will generate double stranded DNA through amplification primers
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 161
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 161
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 161
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 161
- 1000COMPATIBLE WITH RFC[1000]