Difference between revisions of "Part:BBa K4897001"

Revision as of 02:39, 4 October 2023

BS DNA-162 (BS DNA 2.0) using in L-RCA for detecting P. acne

BS DNA-160 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. Binding region is the key element in reacting with P. acne 16s rRNA gene. The DNA ligase will perform the ligation of the single strand DNA meanwhile the phi29 will generate double stranded DNA through amplification primers

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 83
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 57
Illegal SpeI site found at 83
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 68
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 83
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 83
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4897001 short</partinfo>
-BS DNA-50 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. Binding region is the key element in reacting with P. acne 16s rRNA gene. The DNA ligase will perform the ligation of the single strand DNA meanwhile the phi29 will generate double stranded DNA through amplification primers
+BS DNA-160 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. Binding region is the key element in reacting with P. acne 16s rRNA gene. The DNA ligase will perform the ligation of the single strand DNA meanwhile the phi29 will generate double stranded DNA through amplification primers
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