Difference between revisions of "Part:BBa K206000:Design"

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===References===
 
===References===
 +
Niland P, Hühne R, Müller-Hill B. (1996). How AraC Interacts Specifically with its Target DNAs. ''J. Mol. Biol.'' 264:667-674. [http://www.ncbi.nlm.nih.gov/pubmed/8980677]

Revision as of 05:07, 18 October 2009

pBAD strong


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There were no special design considerations.


Source

Site-directed mutagenesis was performed on BBa_I13453 using the following primers:
Forward: TAATCTTATGGACTATCTTGCTATGGCATAGC
Reverse: GCGGATCCTACCTGACGCTTTTTATC

References

Niland P, Hühne R, Müller-Hill B. (1996). How AraC Interacts Specifically with its Target DNAs. J. Mol. Biol. 264:667-674. [http://www.ncbi.nlm.nih.gov/pubmed/8980677]