Difference between revisions of "Part:BBa K4839006"

Latest revision as of 17:27, 3 October 2023

vhhGFP4 (GFP Antibody frgement)

We firstly make use of the vhhGFP4, an efficient anti-GFP antibody peptide as our preliminary validation. vhhGFP4 can specifically target to the GFP-IRF4 protein, and then the SPOP component will recruit the ubiquitin and initiate the ubiquitination of GFP-IRF4, which will subsequently led to the degradation of this target protein (Figure 1). We prove this process through our experiment.

Figure1. The function of anti-GFP BioPROTAC

[1] Lim S , Khoo R , Peh K M ,et al.bioPROTACs as versatile modulators of intracellular therapeutic targets including proliferating cell nuclear antigen (PCNA)[J].Proceedings of the National Academy of Sciences, 2020(11).DOI:10.1073/PNAS.1920251117.

[2] Békés, M., Langley, D.R. & Crews, C.M. PROTAC targeted protein degraders: the past is prologue. Nat Rev Drug Discov 21, 181–200 (2022). https://doi.org/10.1038/s41573-021-00371-6

Sequence and Features