Difference between revisions of "Part:BBa K206000"

Revision as of 04:02, 18 October 2009

pBAD strong

Usage and Biology

pBAD is an E.coli promoter that is induced by L-arabinose. In the absence of arabinose, the repressor protein AraC (BBa_I13458) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription (see Figure 1). In the presence of arabinose, AraC binds to it and changes its conformation such that it interacts with the AraI1 and AraI2 operator sites, permitting transcription (Figure 1).

Figure 1. AraC induction by L-arabinose. [http://www.ncbi.nlm.nih.gov/pubmed/8980677 Reference.]

K206000 is a pBAD promoter with a variant AraI1 site that has been shown to be responsive to lower concentrations of arabinose than the wild type (BBa_I13453); additionally, it exhibits a higher maximum of protein expression, as measured by coupling to a fluorescent reporter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. In the absence of arabinose, the repressor protein AraC (<partinfo>I13458</partinfo>) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription (see Figure 1). In the presence of arabinose, AraC binds to it and changes its conformation such that it interacts with the AraI1 and AraI2 operator sites, premitting transcription (Figure 1).
+pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. In the absence of arabinose, the repressor protein AraC (<partinfo>I13458</partinfo>) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription (see Figure 1). In the presence of arabinose, AraC binds to it and changes its conformation such that it interacts with the AraI1 and AraI2 operator sites, permitting transcription (Figure 1).
 [[Image:BritishColumbia-AraC.jpg‎|center|200px|thumb|Figure 1. AraC induction by L-arabinose. [http://www.ncbi.nlm.nih.gov/pubmed/8980677 Reference.]]]