Difference between revisions of "Part:BBa K206000:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K206000 short</partinfo>
 
<partinfo>BBa_K206000 short</partinfo>
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===Source===
 
===Source===
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Site-directed mutagenesis was performed on <partinfo>I13453</partinfo> using the following primers:<br>
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The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
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Forward: TAATCTTATGGACTATCTTGCTATGGCATAGC<br>
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Reverse: GCGGATCCTACCTGACGCTTTTTATC
  
 
===References===
 
===References===

Revision as of 03:42, 18 October 2009

pBAD strong


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There were no special design considerations.


Source

Site-directed mutagenesis was performed on BBa_I13453 using the following primers:
Forward: TAATCTTATGGACTATCTTGCTATGGCATAGC
Reverse: GCGGATCCTACCTGACGCTTTTTATC

References