Difference between revisions of "Part:BBa K4634000"

Revision as of 14:59, 3 October 2023

pPepT: hypoxia-inducible promoter

pPepT, which is induced by hypoxia, is regulated by transcriptional activator: fumarate and nitrate reduction regulatory protein (FNR).[1]Under anaerobic conditions, FNR associates with [4Fe-4S]2+ clusters to form transcriptionally active homodimers, the dimer can bind DNA site-specifically and regulate transcription from target promoters. However, when the clusters are disrupted in the presence of oxygen, the FNR dimer dissociates into nonDNA-binding monomers. [2, 3]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4634000 short</partinfo>
-The pET28a vector is a molecular biology tool specifically designed for the efficient expression of recombinant proteins in bacterial host cells. Unlike ordinary pET28a vector, this vector lacks the T7 promoter and lac operon, it is a vector used to construct other induced expression systems. Additionally, the vector carries a resistance marker, typically kanamycin resistance, for the selection of transformed bacterial cells, and a high-copy-number origin of replication compatible with Escherichia coli, ensuring efficient plasmid replication. Other than that, this plasmid backbone contains a rop protein which maintains plasmids at low copy number.
+pPepT, which is induced by hypoxia, is regulated by transcriptional activator: fumarate and nitrate reduction regulatory protein (FNR).[1]Under anaerobic conditions, FNR associates with [4Fe-4S]2+ clusters to form transcriptionally active homodimers, the dimer can bind  DNA  site-specifically  and  regulate  transcription  from  target promoters. However, when the clusters are disrupted in the presence of oxygen, the FNR dimer dissociates into nonDNA-binding monomers. [2, 3]
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