Difference between revisions of "Part:BBa K4839007"

Revision as of 14:32, 3 October 2023

SPOP ( speckled POZ protein )

SPOP, speckled POZ protein, is an E3 adapter protein. In our experiment, we proved that the direct conjunction between vhhGFP4 and SPOP can lead to the degradation of GFP-IRF4 protein.

Figure1.The function of anti-GFP BioPROTAC

Just as Figure1 shows, the SPOP act as the backbone of the BioPROTAC, the vhhGFP can be changed into any other pretein binding region, and thus it can also degrade other proteins. The function of SPOP relies on the subunit that binds to the ubiquitin and transform it to the target protein. Once the target protein is become ubiquitination, it will soon be degraded by the proteasomes. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 127
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 508
Illegal SapI.rc site found at 522

@@ Line 4: / Line 4: @@
 SPOP, speckled POZ protein, is an E3 adapter protein. In our experiment, we proved that the direct conjunction between vhhGFP4 and SPOP can lead to the degradation of GFP-IRF4 protein.
+<div style="text-align: center;">
+<html><img src="https://static.igem.wiki/teams/4839/wiki/parts-files/6-1.png" width="650"</html>
+</div>
+<p align="center">Figure1.The function of anti-GFP BioPROTAC</p>
+Just as Figure1 shows, the SPOP act as the backbone of the BioPROTAC, the vhhGFP can be changed into any other pretein binding region, and thus it can also degrade other proteins. The function of SPOP relies on the subunit that binds to the ubiquitin and transform it to the target protein. Once the target protein is become ubiquitination, it will soon be degraded by the proteasomes.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===