Difference between revisions of "Part:BBa K4601051"
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<partinfo>BBa_K4601051 short</partinfo> | <partinfo>BBa_K4601051 short</partinfo> | ||
− | + | This part is CmR gene coding for the chloramphenicol ''O''-acetyltransferase enzyme (Uniprot P62577). | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | The chloramphenicol ''O''-acetyltransferase enzyme (EC 2.3.1.28) catalyses the conversion of chloramphenicol into chloramphenicol 3-acetate (Figure 1), thus inactivating the antibiotic. | ||
+ | |||
+ | [[File: T--Evry_Paris-Saclay--beta-lactam_ring_hydrolysis.png|center|300px]] | ||
+ | |||
+ | Figure 1. The mode of action of the chloramphenicol ''O''-acetyltransferase enzyme: acetylation of chloramphenicol. | ||
+ | |||
+ | The CmR gene was isolated from the bacteriophage P1Cm carrying the Tn''cam''-204 transposon derived from the R plasmid NR1 [1]. | ||
+ | |||
+ | |||
+ | ===Reference=== | ||
+ | [1] Marcoli R, Iida S, Bickle TA. The DNA sequence of an IS/-flanked transposon coding for resistance to chloramphenicol and fusidic acid. FEBS letters (1980) 110: 11–14. | ||
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Revision as of 11:59, 3 October 2023
CmR
This part is CmR gene coding for the chloramphenicol O-acetyltransferase enzyme (Uniprot P62577).
Usage and Biology
The chloramphenicol O-acetyltransferase enzyme (EC 2.3.1.28) catalyses the conversion of chloramphenicol into chloramphenicol 3-acetate (Figure 1), thus inactivating the antibiotic.
Figure 1. The mode of action of the chloramphenicol O-acetyltransferase enzyme: acetylation of chloramphenicol.
The CmR gene was isolated from the bacteriophage P1Cm carrying the Tncam-204 transposon derived from the R plasmid NR1 [1].
Reference
[1] Marcoli R, Iida S, Bickle TA. The DNA sequence of an IS/-flanked transposon coding for resistance to chloramphenicol and fusidic acid. FEBS letters (1980) 110: 11–14.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]