Difference between revisions of "Part:BBa K4937025"

Latest revision as of 11:28, 3 October 2023

TEF1p-SPE3-PRM9t

This part is used to construct the plasmid library that can stably express SPE2 and tune the expression level of SPE3 and AtACL5 to fully use the spermidine substrate and examine the numerical relationship between spermidine and thermospermine.

We constructed this part by two round PCR. We firstly achieved all short single fragment by PCR from the genome of S. cerevisiae and plasmid (Figure 1). Then we connected all single expression device by fusion PCR (Figure 2).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1346
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 167

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4937025 short</partinfo>
+<p>This part is used to construct the plasmid library that can stably express SPE2 and tune the expression level of SPE3 and AtACL5 to fully use the spermidine substrate and examine the numerical relationship between spermidine and thermospermine.</p>
+https://static.igem.wiki/teams/4937/wiki/part/24-1.png
+<p>We constructed this part by two round PCR. We firstly achieved all short single fragment by PCR from the genome of <i>S. cerevisiae</i> and plasmid (Figure 1). Then we connected all single expression device by fusion PCR (Figure 2). </p>
+https://static.igem.wiki/teams/4937/wiki/part/24-2.png
+https://static.igem.wiki/teams/4937/wiki/part/24-3.png
-<p>This part was used to constitutively express SPE3 by TEF1p, and it was integrated with BBa_K4937024 and BBa_K4937030/BBa_K4937031/BBa_K4937032/BBa_K4937033 to enhance the flux of polyamine synthesis in <i>Saccharomyces cerevisiae</i>, and also the product of thermospermine, which may confer thermotolerance to strain.</p>
 <!-- Add more about the biology of this part here