Difference between revisions of "Part:BBa K228004"

Revision as of 23:53, 17 October 2009

NahR( reverse) - salicylate promoter

Designed by Lin Min Group: iGEM09_PKU_Beijing (2009-09-18)
Input: Salicylate molecules
Output: GFP fluorescence

Part Main Page

Transfer Function

Growth Rate

'Description=

This is a salicylate inducible promoter part constructed by Lin Min. The activator protein (NahR) gene is in the upstream but in an opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated. If we place a generator on its downstream, the transcription of the coding sequence will be triggered by the salicylate-binded positive transcriptional activator protein which is encoded by NahR.

It is necessary to notice that there is no terminator downstream of the NahR coding sequence, but it has no effect son parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unexpected expression.

For the purpose of characterization, we connected BBa_E0840 downstream of the salicylate promoter to make the composite part, BBa_K228850. This report system allowed us to test the salicylate inducible promoter indirectly via the fluorescence output of BBa_E0840, while the input is salicylate solution at a gradient of concentration.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 576
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 408
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K228004 short</partinfo>
-This is a salicylate inducible promoter part.
+Designed by Lin Min   Group: iGEM09_PKU_Beijing   (2009-09-18)<br>
-The activator protein is right upstream but in a opposite direction of the regulated promoter.
+''Input'': Salicylate molecules<br>
-In the presence of salicylate, the promoter can be activated.
+''Output'': GFP fluorescence<br>
+{|cellpadding=3
+|   Part Main Page  ||  Transfer Function   ||  Growth Rate
+|}
+=='''Description''===
+This is a salicylate inducible promoter part constructed by Lin Min. The activator protein (NahR) gene is in the upstream but in an opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated. If we place a generator on its downstream, the transcription of the coding sequence will be triggered by the salicylate-binded positive transcriptional activator protein which is encoded by NahR.
+It is necessary to notice that there is no terminator downstream of the NahR coding sequence, but it has no effect son parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unexpected expression.
+For the purpose of characterization, we connected BBa_E0840 downstream of the salicylate promoter to make the composite part, BBa_K228850. This report system allowed us to test the salicylate inducible promoter indirectly via the fluorescence output of BBa_E0840, while the input is salicylate solution at a gradient of concentration.
-Note:
-There is no termoinator downstream of the NahR coding sequence, but it has no effect on parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unwanted expression.
-This promoter is then placed upstream of a GFP gene to get it induction data. We use flowcytometry to test the fluorescence at different concentrations of the inducer salicylate.
-The Induction curve is shown below:
 [[Image:PKU_Salicylate.jpg|600px]]