Difference between revisions of "Part:BBa K4601041:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
to do
 
  
 +
To disrupt the BsaI recognition site GGTCTC by site-directed mutagenesis we choose to change its sequence to GGTCGC. Care was taken that the mutation should not affect the amino acid composition of the protein. The chosen mutation is a synonymous one TCT(Ser) to TCG(Ser).
  
 +
                      >BsaI
 +
                      |
 +
pSB1A3:      1171 - GGG TCT CGC - 1179
 +
                      G  S  R
 +
 +
BBa K4601041:  715 - GGG TCG CGC - 723
 +
                      G  S  R
  
−
===Source===
 
  
−
to do
+
===Source===
  
−
===References===
+
PCR amplification from a BsaI-free version of pSB1A3

Latest revision as of 20:23, 2 October 2023


AmpR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To disrupt the BsaI recognition site GGTCTC by site-directed mutagenesis we choose to change its sequence to GGTCGC. Care was taken that the mutation should not affect the amino acid composition of the protein. The chosen mutation is a synonymous one TCT(Ser) to TCG(Ser). 

                     >BsaI
                      |
pSB1A3:       1171 - GGG TCT CGC - 1179
                      G   S   R

BBa K4601041:  715 - GGG TCG CGC - 723
                      G   S   R


Source

PCR amplification from a BsaI-free version of pSB1A3