Difference between revisions of "Part:BBa K4759002"
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<partinfo>BBa_K4759003 short</partinfo>
 
a gene coding for protein PdR(CamA), CamA is a putidaredoxin reductase. In the camphor monooxygenase system from Pseudomonas putida, the containing putidaredoxin (Pdx) shuttles electrons between the NADH-dependent putidaredoxin reductase (Pdr) and cytochrome P450(cam). 
 
a gene coding for protein PdR(CamA), CamA is a putidaredoxin reductase. In the camphor monooxygenase system from Pseudomonas putida, the containing putidaredoxin (Pdx) shuttles electrons between the NADH-dependent putidaredoxin reductase (Pdr) and cytochrome P450(cam). 
 
this sequence has been codon-optimized for expression in Escherichia coli.  
 
this sequence has been codon-optimized for expression in Escherichia coli.  
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Optimizing protein to protein interactions using different methods to improve the electron transfer efficiency of the P450 system, known as "redox chaperone engineering", is one of the important means of engineering P450s, and fruitful progress has been made.
 
Optimizing protein to protein interactions using different methods to improve the electron transfer efficiency of the P450 system, known as "redox chaperone engineering", is one of the important means of engineering P450s, and fruitful progress has been made.
 
Several studies have confirmed that the combined expression of P450, enzyme with different RPs can achieve the reconstruction and promotion of reactivity. This strategy has been widely used in the bacterial class I P450 system.
 
Several studies have confirmed that the combined expression of P450, enzyme with different RPs can achieve the reconstruction and promotion of reactivity. This strategy has been widely used in the bacterial class I P450 system.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4759003 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4759003 parameters</partinfo>
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Revision as of 17:11, 2 October 2023


camB a gene coding for protein PdR(CamA), CamA is a putidaredoxin reductase. In the camphor monooxygenase system from Pseudomonas putida, the containing putidaredoxin (Pdx) shuttles electrons between the NADH-dependent putidaredoxin reductase (Pdr) and cytochrome P450(cam).  this sequence has been codon-optimized for expression in Escherichia coli. The P450 enzymes are redox-dependent proteins, through which they source electrons from reducing cofactors to drive their activities. In bacterial systems, the electrostatic interactions between the ferredoxin (Fdx), P450 heme and the reductase domains, as well as the negatively and positively charged amino acids on the Fdx iron-sulfur cluster and P450 proximal site, mediate the conformational changes of the Fdx for electron transfer to P450s. In addition, the substrate binding to P450 induces P450 conformational change to increase its preference for Fdx through electrostatic and steric complementarity . Optimizing protein to protein interactions using different methods to improve the electron transfer efficiency of the P450 system, known as "redox chaperone engineering", is one of the important means of engineering P450s, and fruitful progress has been made. Several studies have confirmed that the combined expression of P450, enzyme with different RPs can achieve the reconstruction and promotion of reactivity. This strategy has been widely used in the bacterial class I P450 system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]