Difference between revisions of "Part:BBa K4806100:Design"
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<partinfo>BBa_K4806100 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4806100 SequenceAndFeatures</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This part has been codon optimized for <i>Chlamydomonas reinhardtii</i> and it is compatible with Phytobricks/MoClo parts. | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | <html> | |
| − | a | + | <p> |
| + | This composite part is a combination of the following parts: | ||
| + | <ul> | ||
| + | <li>1. PSAD-promotor (A1-B2) (<a href=" https://parts.igem.org/Part:BBa_K3002001">BBa_K3002001</a>)<sup>*</sup></li> | ||
| + | <li>2. Hygromycin resistance gene (B3-B5) (<a href=" https://parts.igem.org/Part:BBa_K4806015">BBa_K4806015</a>)</li> | ||
| + | <li>3. PSAD-terminator (B6-C1) (<a href=" https://parts.igem.org/Part:BBa_K3002002">BBa_K3002002</a>)<sup>*</sup></li> | ||
| + | </ul> | ||
| + | Additionally, connectors were used to create the MoClo overhangs: | ||
| + | <ul> | ||
| + | <li>1. Connector B2-B3 (<a href=" https://parts.igem.org/Part:BBa_K3002303">BBa_K3002303</a>)<sup>*</sup></li> | ||
| + | <li>2. Connector B5-B6 (<a href=" https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>)<sup>*</sup></li> | ||
| + | </ul> | ||
| + | </p> | ||
| + | <p>The <sup>*</sup> marked parts were not created by us. Our results can be found on the experience page of each part.</p> | ||
| + | </html> | ||
===References=== | ===References=== | ||
Latest revision as of 11:16, 2 October 2023
Hygromycin Resistance for Chlamydomonas reinhardtii (Phytobrick)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1270
Illegal NgoMIV site found at 1452 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.
Source
This composite part is a combination of the following parts:
- 1. PSAD-promotor (A1-B2) (BBa_K3002001)*
- 2. Hygromycin resistance gene (B3-B5) (BBa_K4806015)
- 3. PSAD-terminator (B6-C1) (BBa_K3002002)*
- 1. Connector B2-B3 (BBa_K3002303)*
- 2. Connector B5-B6 (BBa_K3002305)*
The * marked parts were not created by us. Our results can be found on the experience page of each part.