Difference between revisions of "Part:BBa K4765025"

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===Usage and Biology===
 
===Usage and Biology===
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We optimized the codons of wildtype Rv Dsup for ’’E. coli’’ K12 and heterologously expressed it in ’’E. coli’’ to enhance its survival rate under UV radiation.
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We optimized the codons of wildtype Rv Dsup for ''E. coli'' K12 and heterologously expressed it in ’’E. coli’’ to enhance its survival rate under UV radiation.
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===Characterization===
 
===Characterization===
  

Revision as of 07:15, 2 October 2023


Rv Dsup codon optimized

contributed by Fudan iGEM 2023

Introduction

Rv Dsup is a damage suppressor protein from the Tardigrade Species Ramazzottius Varieornatus [1] . This is a nucleosome-binding protein that protects chromatin from hydroxyl radicals[2] , thereby inhibiting DNA molecule breakage. This protein can reduce X-ray-induced damage in human cells by 40%.

Usage and Biology

We optimized the codons of wildtype Rv Dsup for E. coli K12 and heterologously expressed it in ’’E. coli’’ to enhance its survival rate under UV radiation.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 169
    Illegal AgeI site found at 240
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 166
    Illegal SapI.rc site found at 895


Reference

  1. ↑ Hashimoto, T., Horikawa, D. D., Saito, Y., Kuwahara, H., Kozuka-Hata, H., Shin-I, T., Minakuchi, Y., Ohishi, K., Motoyama, A., Aizu, T., Enomoto, A., Kondo, K., Tanaka, S., Hara, Y., Koshikawa, S., Sagara, H., Miura, T., Yokobori, S. I., Miyagawa, K., Suzuki, Y., … Kunieda, T. (2016). Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nature communications, 7, 12808. https://doi.org/10.1038/ncomms12808
  2. ↑ Chavez, C., Cruz-Becerra, G., Fei, J., Kassavetis, G. A., & Kadonaga, J. T. (2019). The tardigrade damage suppressor protein binds to nucleosomes and protects DNA from hydroxyl radicals. eLife, 8, e47682. https://doi.org/10.7554/eLife.47682