Difference between revisions of "Part:BBa K4765009"
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===Introduction=== | ===Introduction=== | ||
| − | LCA(256AA, 30kD)，''Lens Culinaris'' Agglutinin, is a lectin from lentils that can recognize α-linked mannose residues. It is a common lectin that can specifically bind to the LPS on the surface of ''S. elongatus'' PCC7942<ref>Arai, S., Okochi, M., Shimizu, K., Hanai, T., & Honda, H. (2016). A single cell culture system using lectin-conjugated magnetite nanoparticles and magnetic force to screen mutant cyanobacteria. ''Biotechnology and Bioengineering, 113''(1), 112–119. https://doi.org/10.1002/bit.25707</ref>. | + | LCA (256AA, 30kD)，''Lens Culinaris'' Agglutinin, is a lectin from lentils that can recognize α-linked mannose residues. It is a common lectin that can specifically bind to the LPS on the surface of ''S. elongatus'' PCC7942<ref>Arai, S., Okochi, M., Shimizu, K., Hanai, T., & Honda, H. (2016). A single cell culture system using lectin-conjugated magnetite nanoparticles and magnetic force to screen mutant cyanobacteria. ''Biotechnology and Bioengineering, 113''(1), 112–119. https://doi.org/10.1002/bit.25707</ref>. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 06:46, 2 October 2023
LCA
Introduction
LCA (256AA, 30kD)，Lens Culinaris Agglutinin, is a lectin from lentils that can recognize α-linked mannose residues. It is a common lectin that can specifically bind to the LPS on the surface of S. elongatus PCC7942[1].
Usage and Biology
In our project, the surface display of LCA lectin on E.coli can mediate adhesion between E. coli and S. elongatus PCC 7942.We fused LCA with intimin, constructing K4765110 ribozyme+strong RBS+intimin-LCA fusion+stem-loop.LCA was outwardly oriented towards the medium for recognition and binding with S. elongatus PCC7942.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
- ↑ Arai, S., Okochi, M., Shimizu, K., Hanai, T., & Honda, H. (2016). A single cell culture system using lectin-conjugated magnetite nanoparticles and magnetic force to screen mutant cyanobacteria. Biotechnology and Bioengineering, 113(1), 112–119. https://doi.org/10.1002/bit.25707