Difference between revisions of "Part:BBa K4765126"
(→‎Characterization)
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This composite part combines [https://parts.igem.org/Part:BBa_K4765016 BBa_K4765016(''H. ex'' mtSSB)],[https://parts.igem.org/Part:BBa_K2306003 BBa_K2306003(SAHS 33020)]and [https://parts.igem.org/Part:BBa_K4765015 BBa_K4765015(AnAFP)] in our ribozyme-assisted polycistronic co-expression system：pRAP.
 
This composite part combines [https://parts.igem.org/Part:BBa_K4765016 BBa_K4765016(''H. ex'' mtSSB)],[https://parts.igem.org/Part:BBa_K2306003 BBa_K2306003(SAHS 33020)]and [https://parts.igem.org/Part:BBa_K4765015 BBa_K4765015(AnAFP)] in our ribozyme-assisted polycistronic co-expression system：pRAP.
 
===Characterization===
 
===Characterization===
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See more in [https://parts.igem.org/Part:BBa_K4765016 BBa_K4765016(''H. ex'' mtSSB)],[https://parts.igem.org/Part:BBa_K2306003 BBa_K2306003(SAHS 33020)]and [https://parts.igem.org/Part:BBa_K4765015 BBa_K4765015(AnAFP)]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 06:44, 2 October 2023


ribozyme connected: H. ex mtSSB + SAHS 33020 + AnAFP contributed by Fudan iGEM 2023


Usage and Biology

This composite part combines BBa_K4765016(H. ex mtSSB),BBa_K2306003(SAHS 33020)and BBa_K4765015(AnAFP) in our ribozyme-assisted polycistronic co-expression system：pRAP.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 880
    Illegal BsaI.rc site found at 515
    Illegal BsaI.rc site found at 596
    Illegal SapI site found at 409
    Illegal SapI.rc site found at 1804