Difference between revisions of "Part:BBa K4765122"

 
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<partinfo>BBa_K4765122 short</partinfo>
 
<partinfo>BBa_K4765122 short</partinfo>
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html>
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__TOC__
  
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ribozyme+RBS+CDS+stem-loop module: pgmA + galU + mScarlet
 
  
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<!-- Add more about the biology of this part here
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===Introduction===
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This composite part is designed for the production of exopolysaccharide(EPS), and detection of EPS’s adhesion ability. It includes ’’E. coli’’ pgmA on the downstream site and ''E. coli'' galU on the upstream site, two essential enzymes in EPS biosynthesis pathway. These two enzymes are tested by iGEM20_XJTU-China in [https://parts.igem.org/Part:BBa_K3331001 E.coli galU]and [https://parts.igem.org/Part:BBa_K3331002 E.coli pgmA]. The following mScarlet is utilized to detection E. coli with adhesion ability.
 
===Usage and Biology===
 
===Usage and Biology===
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We use this part to improve EPS production of ''E. coli'' and assessment of EPS’s adhesion ability.
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===Characterization===
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Revision as of 17:59, 1 October 2023


ribozyme connected: pgmA + galU + mScarlet contributed by Fudan iGEM 2023


Introduction

This composite part is designed for the production of exopolysaccharide(EPS), and detection of EPS’s adhesion ability. It includes ’’E. coli’’ pgmA on the downstream site and E. coli galU on the upstream site, two essential enzymes in EPS biosynthesis pathway. These two enzymes are tested by iGEM20_XJTU-China in E.coli galUand E.coli pgmA. The following mScarlet is utilized to detection E. coli with adhesion ability.

Usage and Biology

We use this part to improve EPS production of E. coli and assessment of EPS’s adhesion ability.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3285
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]