Difference between revisions of "Part:BBa K4621020"
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Modified Streptomyces SCUT-3. They were then inoculated in a 40 mL LB medium system. After fermentation at 40 ° C and 220 rpm for 72 hours, the product GABA was measured, and the results were as follows: | Modified Streptomyces SCUT-3. They were then inoculated in a 40 mL LB medium system. After fermentation at 40 ° C and 220 rpm for 72 hours, the product GABA was measured, and the results were as follows: | ||
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[WT GABA Gi1 3 9 fermentation data graph] | [WT GABA Gi1 3 9 fermentation data graph] | ||
Revision as of 17:22, 1 October 2023
gadB is the coding sequence of glutamate decarboxylase, which converts glutamate to 4-Aminobutanoate
Usage, Biology and Characterization：
gadB is the coding sequence for glutamate decarboxylase, GAD is a pyridoxal 5'phosphate (PLP)-dependent decarboxylase widespread in bacteria yeast and filamentous fungi. The carboxyl group (-COOH) on the catalytic glutamate molecule is released to CO2, and 4-Aminobutanoate is generated at the same time, which participates in the utilization of α-Ketoglutaricacid in the TCA cycle. The specific synthesis route is shown in FIG. 1.
Fig.1 Biosynthetic pathway of GABA
Modified Streptomyces SCUT-3. They were then inoculated in a 40 mL LB medium system. After fermentation at 40 ° C and 220 rpm for 72 hours, the product GABA was measured, and the results were as follows:
[WT GABA Gi1 3 9 fermentation data graph]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 53
Illegal NgoMIV site found at 1242
Illegal AgeI site found at 470
Illegal AgeI site found at 690 - 1000COMPATIBLE WITH RFC[1000]