Difference between revisions of "Part:BBa K4727114"

 
 
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<partinfo>BBa_K4727114 short</partinfo>
 
<partinfo>BBa_K4727114 short</partinfo>
  
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This sequence has been designed with bioinformatic tools based on the analysis of the genomic sequence of Klebsiella pneumoniae.
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This guide DNA has been designed to work with dCas9 to target AMR related genes in <i>Acinetobacter baumannii </i>. The guide binds 98bp downstream the TSS, on the RV strand of the gene.
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The AlgC gene was studied in <i>Acinetobacter baumannii</i> AIIMS 7 [1], a multidrug resistant strain, as a candidate gene in biofilm production. Further analysis identified the transcription product as the bifunctional enzyme phosphomannomutase/
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phosphoglucomutase, necessary to produce exopolysaccharide core in alginate and lipopolysaccharide core. In particular, this enzyme would have a similar structure to that of <i>P. aeruginosa</i>. Transcript levels appear to be in close association with biofilm formation, particularly with the maturation phase (36–48 hours) and also with the initial attachment phase (3–9 hours).
  
 
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<partinfo>BBa_K4727114 parameters</partinfo>
 
<partinfo>BBa_K4727114 parameters</partinfo>
 
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===References===
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[1] Sahu PK, Iyer PS, Barage SH, Sonawane KD, Chopade BA. Characterization of the algC gene expression pattern in the multidrug resistant Acinetobacter baumannii AIIMS 7 and correlation with biofilm development on abiotic surface. ScientificWorldJournal. 2014;2014:593546. doi: 10.1155/2014/593546.

Latest revision as of 16:30, 1 October 2023


sgDNA AlgC RV 98

This guide DNA has been designed to work with dCas9 to target AMR related genes in Acinetobacter baumannii . The guide binds 98bp downstream the TSS, on the RV strand of the gene.

The AlgC gene was studied in Acinetobacter baumannii AIIMS 7 [1], a multidrug resistant strain, as a candidate gene in biofilm production. Further analysis identified the transcription product as the bifunctional enzyme phosphomannomutase/ phosphoglucomutase, necessary to produce exopolysaccharide core in alginate and lipopolysaccharide core. In particular, this enzyme would have a similar structure to that of P. aeruginosa. Transcript levels appear to be in close association with biofilm formation, particularly with the maturation phase (36–48 hours) and also with the initial attachment phase (3–9 hours).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

[1] Sahu PK, Iyer PS, Barage SH, Sonawane KD, Chopade BA. Characterization of the algC gene expression pattern in the multidrug resistant Acinetobacter baumannii AIIMS 7 and correlation with biofilm development on abiotic surface. ScientificWorldJournal. 2014;2014:593546. doi: 10.1155/2014/593546.