Difference between revisions of "Part:BBa K4645000"

Revision as of 16:14, 1 October 2023

FelD: Felis domesticus allergen

Recombinant FelD, produced by E. coli, exhibits comparable immunogenicity to Fel d 1 extracted from cats and is capable of inducing allergies in the human body. Its structure comprises the 93 amino acids of chain 2 of natural FelD that is directly fused to the 70 amino acids of chain 1. This is followed by the addition of 6*His tag and Flag tag to the end of the C-terminal. By binding with IgE in individuals allergic to cats, FelD mediates allergy symptoms in the affected individuals. In our study, FelD was utilized to investigate the blocking effect of our scFvs (single-chain fragment variables) on cat allergies.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 467
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4645000 short</partinfo>
-Recombinant FelD, produced by E. coli, exhibits comparable immunogenicity to Fel d 1 extracted from cats and is capable of inducing allergies in the human body. Its structure comprises the 93 amino acids of chain 2 of natural FelD that is directly fused to the 70 amino acids of chain 1. This is followed by the addition of 6*His tag and Flag tag to the end of the C-terminal. By binding with IgE in individuals allergic to cats, FelD mediates allergy symptoms in the affected individuals. In our study, FelD was utilized to investigate the blocking effect of our scFvs (single-chain fragment variables) on cat allergies.
+Recombinant FelD, produced by <i>E. coli</i>, exhibits comparable immunogenicity to Fel d 1 extracted from cats and is capable of inducing allergies in the human body. Its structure comprises the 93 amino acids of chain 2 of natural FelD that is directly fused to the 70 amino acids of chain 1. This is followed by the addition of 6*His tag and Flag tag to the end of the C-terminal. By binding with IgE in individuals allergic to cats, FelD mediates allergy symptoms in the affected individuals. In our study, FelD was utilized to investigate the blocking effect of our scFvs (single-chain fragment variables) on cat allergies.
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