Difference between revisions of "Part:BBa K4765111"

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===Introduction===
 
===Introduction===
 
This composite part is composed of AnAFP and ribozyme-assisted polycistronic co-expression system：pRAP. AnAFP is an antifreeze protein derived from ''Ammopiptanthus nanus''. It protects the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions
 
This composite part is composed of AnAFP and ribozyme-assisted polycistronic co-expression system：pRAP. AnAFP is an antifreeze protein derived from ''Ammopiptanthus nanus''. It protects the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions
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<ref>Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang,  S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of  antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold  tolerance in ''Escherichia coli'' and tobacco. ’‘Gene, 539’‘(1), 132-140.https://doi.org/10.1016/j.gene.2014.01.013</ref>.
+
<ref>Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang,  S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of  antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold  tolerance in ''Escherichia coli'' and tobacco. ''Gene, 539''(1), 132-140.https://doi.org/10.1016/j.gene.2014.01.013</ref>.
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 15:06, 1 October 2023


Twister P1 + T7_RBS + AnAFP + stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of AnAFP and ribozyme-assisted polycistronic co-expression system：pRAP. AnAFP is an antifreeze protein derived from Ammopiptanthus nanus. It protects the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions [1].

Usage and Biology

The heterologously expressed codon-optimized AnAFP can endowing E.coli, with antifreeze capability.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 512


Reference

  1. ↑ Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang, S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in Escherichia coli and tobacco. Gene, 539(1), 132-140.https://doi.org/10.1016/j.gene.2014.01.013