Difference between revisions of "Part:BBa K4765015"
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===Introduction=== | ===Introduction=== | ||
− | ''Ammopiptanthus nanus'' antifreeze protein (AnAFP) is an antifreeze protein derived from ''Ammopiptanthus nanus''. It protects the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions<ref>Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang, S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in ''Escherichia coli'' and tobacco. | + | ''Ammopiptanthus nanus'' antifreeze protein (AnAFP) is an antifreeze protein derived from ''Ammopiptanthus nanus''. It protects the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions<ref>Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang, S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of antifreeze protein gene AnAFP from ''Ammopiptanthus nanus'' enhances cold tolerance in ''Escherichia coli'' and tobacco. ''Gene, 539''(1), 132-140.https://doi.org/10.1016/j.gene.2014.01.013</ref>. |
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===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 14:53, 1 October 2023
AnAFP
Introduction
Ammopiptanthus nanus antifreeze protein (AnAFP) is an antifreeze protein derived from Ammopiptanthus nanus. It protects the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions[1].
Usage and Biology
We heterologously expressed codon-optimized AnAFP in E.coli, endowing it with antifreeze capability.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 451
References
- ↑ Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang, S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in Escherichia coli and tobacco. Gene, 539(1), 132-140.https://doi.org/10.1016/j.gene.2014.01.013