Difference between revisions of "Part:BBa K4765119"
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html> | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html> | ||
__TOC__ | __TOC__ | ||
| − | + | ===Introduction=== | |
| + | This composite part inculdes | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
We designed an assessment setup to characterize the performance of pRAP. This setup incorporates Staygold, mScarlet and two adjustable-strength RBS elements. Initially, we employed the assessment setup with the same RBS to validate regular protein expression in pRAP. | We designed an assessment setup to characterize the performance of pRAP. This setup incorporates Staygold, mScarlet and two adjustable-strength RBS elements. Initially, we employed the assessment setup with the same RBS to validate regular protein expression in pRAP. | ||
Revision as of 13:44, 1 October 2023
ribozyme test: constitutive expression
Introduction
This composite part inculdes
Usage and Biology
We designed an assessment setup to characterize the performance of pRAP. This setup incorporates Staygold, mScarlet and two adjustable-strength RBS elements. Initially, we employed the assessment setup with the same RBS to validate regular protein expression in pRAP.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 1399 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 690
Illegal BsaI.rc site found at 710