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Revision as of 12:47, 1 October 2023

Twister P1 + T7_RBS + intimin-MVN fusion + stem-loop

contributed by Fudan iGEM 2023

Introduction

We’ve developed an E. coli-cyanobacteria adhesion module by transfecting intimin-MVN fusion. Intimin-MVN fusion is composed of intimin and MVN. MVN is a lectin isolated from the cyanobacteria Microcystis aeruginosa PCC7806 and it was tested by iGEM14_Peking. Intimin includes a short N-terminal signal peptide to direct its trafficking to the periplasm, a LysM domain for peptidoglycan binding, and a beta-barrel for transmembrane insertion[1] , possess the outer membrane anchoring of MVN. We’ve constructed this fusion protein into our ribozyme-assisted polycistronic co-expression system：pRAP.

Usage and Biology

This biological component delivers MVN to the surface of ‘’E. coli’’, facilitating adhesion between ‘’E. coli’’ and ‘’Microcystis aeruginosa’’ PCC7806. We envision that the adhesion between cyanobacteria and ‘’E. coli’’ can promote the exchange of substances within the biofilm, enhancing the biofilm's survivability.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1305
    Illegal SapI site found at 2058

Reference

  1. ↑ Piñero-Lambea, C., Bodelón, G., Fernández-Periáñez, R., Cuesta, A. M., Álvarez-Vallina, L., & Fernández, L. Á. (2015). Programming controlled adhesion of E. coli to target surfaces, cells, and tumors with synthetic adhesins. ACS Synthetic Biology, 4(4), 463–473. https://doi.org/10.1021/sb500252a