Difference between revisions of "Part:BBa K4765119"

 
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<partinfo>BBa_K4765119 short</partinfo>
 
<partinfo>BBa_K4765119 short</partinfo>
  
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ribozyme measurement module: twister ribozyme
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===Usage and Biology===
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We designed an assessment setup to characterize the performance of pRAP. This setup incorporates Staygold, mScarlet and two adjustable-strength RBS elements. Initially, we employed the assessment setup with the same RBS to validate regular protein expression in pRAP.
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===Characterization===
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:51, 1 October 2023


ribozyme test: constitutive expression

Usage and Biology

We designed an assessment setup to characterize the performance of pRAP. This setup incorporates Staygold, mScarlet and two adjustable-strength RBS elements. Initially, we employed the assessment setup with the same RBS to validate regular protein expression in pRAP.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 1399
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 690
    Illegal BsaI.rc site found at 710