Difference between revisions of "Part:BBa K4765114"

 
Line 3: Line 3:
 
<partinfo>BBa_K4765114 short</partinfo>
 
<partinfo>BBa_K4765114 short</partinfo>
  
−
ribozyme+T7_RBS+Dsup+stem-loop
+
===Introduction===
 +
This composite part is composed of Dsup and ribozyme-assisted polycistronic co-expression system：pRAP. Dsup is a protein that helps protect DNA from radiation damage. It was discovered in the Tardigrade Species Ramazzottius Varieornatus and tested by iGEM17_WashU_StLouis in [https://parts.igem.org/Part:BBa_K2195000  Dsup (Radiation Protection)]
 +
===Usage and Biology===
 +
We constructed Dsup into pRAP to endow ''E. coli'' with anti-UV capability.
 +
===Characterization===
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 17: Line 22:
 
<partinfo>BBa_K4765114 parameters</partinfo>
 
<partinfo>BBa_K4765114 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
==Reference==

Revision as of 08:26, 1 October 2023


Twister P1 + T7_RBS + Rv Dsup + stem-loop

Introduction

This composite part is composed of Dsup and ribozyme-assisted polycistronic co-expression system：pRAP. Dsup is a protein that helps protect DNA from radiation damage. It was discovered in the Tardigrade Species Ramazzottius Varieornatus and tested by iGEM17_WashU_StLouis in Dsup (Radiation Protection)

Usage and Biology

We constructed Dsup into pRAP to endow E. coli with anti-UV capability.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 230
    Illegal AgeI site found at 301
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 227
    Illegal SapI.rc site found at 956


Reference