Difference between revisions of "Part:BBa K4621000:Design"

 
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===References===
 
===References===
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[1] Garcia-Estepa R et al.Sys Appl Microbiol, 2006, 29 (8) :626-633

Latest revision as of 07:57, 1 October 2023


EctOperon is a cluster of genes encoding four enzymes that synthesize 5-Hydroxyectoine


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3227
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3227
    Illegal NotI site found at 571
    Illegal NotI site found at 1456
    Illegal NotI site found at 1774
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 753
    Illegal XhoI site found at 927
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3227
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3227
    Illegal NgoMIV site found at 788
    Illegal NgoMIV site found at 1194
    Illegal NgoMIV site found at 1200
    Illegal NgoMIV site found at 1491
    Illegal NgoMIV site found at 2540
    Illegal NgoMIV site found at 2732
    Illegal NgoMIV site found at 3242
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 376
    Illegal BsaI.rc site found at 556
    Illegal BsaI.rc site found at 1338
    Illegal BsaI.rc site found at 1866
    Illegal BsaI.rc site found at 2121
    Illegal BsaI.rc site found at 2211
    Illegal BsaI.rc site found at 2286
    Illegal SapI site found at 414


Design Notes

The GC content will be taken into account when designing the primers.


Source

This part is derived from SCUT-3 genome.

References

[1] Garcia-Estepa R et al.Sys Appl Microbiol, 2006, 29 (8) :626-633