Difference between revisions of "Part:BBa K4604011:Design"

 
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===Design Notes===
 
===Design Notes===
−
First methionine codon was removed to fit a FLAG-tag in the reading frame.
+
First methionine codon was removed to fit a FLAG-tag in the reading frame. Stop codon was also removed to guarantee translation of reporter gene afterwards.
  
  

Revision as of 15:32, 30 September 2023


MazF toxin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

First methionine codon was removed to fit a FLAG-tag in the reading frame. Stop codon was also removed to guarantee translation of reporter gene afterwards.


Source

Occurs naturally in E. coli K12 MG1655 genome, synthesised by IDT. Sequence taken from National Library of Medizine.

References