Difference between revisions of "Part:BBa K4765110"

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===Usage and Biology===
 
===Usage and Biology===
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This biological component delivers LCA to the surface of ''E. coli'', facilitating adhesion between ''E. coli,, and ''S. elongatus '' PCC7942. We envision that the adhesion between cyanobacteria and ''E. coli'' can promote the exchange of substances within the biofilm, enhancing the biofilm's survivability.
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This biological component delivers LCA to the surface of ''E. coli'', facilitating adhesion between ''E. coli'', and ''S. elongatus '' PCC7942. We envision that the adhesion between cyanobacteria and ''E. coli'' can promote the exchange of substances within the biofilm, enhancing the biofilm's survivability.
  
 
===Characterization===
 
===Characterization===

Revision as of 13:08, 30 September 2023

Twister P1 + T7_RBS + intimin-LCA fusion + stem-loop

Introduction

We’ve developed an E. coli-cyanobacteria adhesion module by transfecting intimin-LCA fusion. Intimin-LCA fusion is composed of intimin and LCA. is a lectin from Lentils that can recognize α-linked mannose residues. It is a common lectin that can specifically bind to the LPS on the surface of S. elongatus PCC7942. Intimin includes a short N-terminal signal peptide to direct its trafficking to the periplasm, a LysM domain for peptidoglycan binding, and a beta-barrel for transmembrane insertion[1] , possess the outer membrane anchoring of LCA. We’ve constructed this fusion protein into our ribozyme-assisted polycistronic co-expression system：pRAP.

Usage and Biology

This biological component delivers LCA to the surface of E. coli, facilitating adhesion between E. coli, and S. elongatus PCC7942. We envision that the adhesion between cyanobacteria and E. coli can promote the exchange of substances within the biofilm, enhancing the biofilm's survivability.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1305

Reference

  1. ↑ Piñero-Lambea, C., Bodelón, G., Fernández-Periáñez, R., Cuesta, A. M., Álvarez-Vallina, L., & Fernández, L. Á. (2015). Programming controlled adhesion of E. coli to target surfaces, cells, and tumors with synthetic adhesins. ACS Synthetic Biology, 4(4), 463–473. https://doi.org/10.1021/sb500252a