Difference between revisions of "Part:BBa K216007"

Revision as of 15:27, 17 October 2009

lumazine protein

This is the lumazine protein of Photobacterium phosphoreum. Lumazine protein (LumP) of Photobacterium phosphoreum is a small protein which binds a fluorescent pteridine, 6,7-dimethyl-8-(1'-D-ribityl)lumazine, as cofactor. In P. phosphoreum, it acts to shift the wavelength of light emitted by luciferase, LuxAB, from 495 nm to a shorter wavelength, around 475 nm (O'Kane et al, 1990 and references cited therein).

Usage and Biology

Lumazine protein (LumP) of Photobacterium phosphoreum is a small protein which binds a fluorescent pteridine, 6,7-dimethyl-8-(1'-D-ribityl)lumazine, as cofactor. In P. phosphoreum, it acts to shift the wavelength of light emitted by luciferase, LuxAB, from 495 nm to a shorter wavelength, around 475 nm (O'Kane et al, 1990 and references cited therein). It may be partially responsible for the much higher perceived brightness of P. phosphoreum luminescence compared to other bioluminescent bacteria such as Xenorhabdus (Photorhabdus) luminescens. To find out, we hope to compare bioluminescence of E. coli expressing X. luminescens luxAB (BBa_K216008) with and without lumazine protein. At the time of writing, days before the freezing of the iGEM 2009 information for judging, we have not yet been able to perform this exciting experiment, but we hope to post the results on the Experience page in the near future (possibly shortly after the Jamboree).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 356
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-Lumazine protein (LumP) of ''Photobacterium phosphoreum'' is a small protein which binds a fluorescent pteridine, 6,7-dimethyl-8-(1'-D-ribityl)lumazine, as cofactor. In ''P. phosphoreum'', it acts to shift the wavelength of light emitted by luciferase, LuxAB, from 495 nm to a shorter wavelength, around 475 nm (O'Kane et al, 1990 and references cited therein). It may be partially responsible for the much higher perceived brightness of P. phosphoreum luminescence compared to other bioluminescent bacteria such as Xenorhabdus luminescens. To find out, we hope to compare bioluminescence of ''E. coli'' expressing ''X. luminescens'' ''luxAB'' (BBa_K216008) with and without lumazine protein. At the time of writing, days before the freezing of the iGEM 2009 information for judging, we have not yet been able to perform this exciting experiment, but we hope to post the results on the Experience page in the near future (possibly shortly after the Jamboree).
+Lumazine protein (LumP) of ''Photobacterium phosphoreum'' is a small protein which binds a fluorescent pteridine, 6,7-dimethyl-8-(1'-D-ribityl)lumazine, as cofactor. In ''P. phosphoreum'', it acts to shift the wavelength of light emitted by luciferase, LuxAB, from 495 nm to a shorter wavelength, around 475 nm (O'Kane et al, 1990 and references cited therein). It may be partially responsible for the much higher perceived brightness of ''P. phosphoreum'' luminescence compared to other bioluminescent bacteria such as ''Xenorhabdus (Photorhabdus) luminescens''. To find out, we hope to compare bioluminescence of ''E. coli'' expressing ''X. luminescens'' ''luxAB'' (BBa_K216008) with and without lumazine protein. At the time of writing, days before the freezing of the iGEM 2009 information for judging, we have not yet been able to perform this exciting experiment, but we hope to post the results on the Experience page in the near future (possibly shortly after the Jamboree).
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